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Part:BBa_J100308:Design

Designed by: Monica Prudencio   Group: Campbell M Lab   (2016-10-10)


Variant of repClone Red(J100205) w/ wt TetR promoter (R0040)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 948
    Illegal SpeI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 9
    Illegal AgeI site found at 2213
    Illegal AgeI site found at 2325
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was constructed using Golden Gate Assembly to ligate the TetR promoter into variant repClone (J100307) following BsaI digestion.



Source

Insertion of R0040 promoter in J100307 (variant repClone).

References